ABSTRACT
AIM To establish an HPLC method for the simultaneous content determination of catalpol,acteoside,jionoside B1,martinoside,calycosin-7-O-β-D-glucoside,ononin,calycosin,formononetin and heterophyllin B in Jiangtangjia Tablets (Rehmanniae Radix,Astragali Radix,Pseudostellariae Radix,etc.).METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic Agilent TC-C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ glacial acetic acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 203,210,254,330 nm.RESULTS Nine constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 96.86％-100.30％ with the RSDs of 0.74％-1.68％.CONCLUSION This stable and reliable method can be used for the quality control of Jiangtangjia Tablets.
ABSTRACT
At the urgent request of Coptis chinensis planting,growth suitability as assessment indicators for C. chinensis cultivation was proposed and analyzed in this paper , based on chemical quality determination and ecological fators analysis by Maxent and ArcGIS model. Its potential distribution areas at differernt suitability grade and regionalization map were formulated based on statistical theory and growth suitability theory. The results showed that the most suitable habitats is some parts of Chongqing and Hubei province, such as Shizhu, Lichuan, Wulong, Wuxi, Enshi. There are seven ecological factor is the main ecological factors affect the growth of Coptidis Rhizoma, including altitude, precipitation in February and September and the rise of precipitation and altitude is conducive to the accumulation of total alkaloid content in C. chinensis. Therefore, The results of the study not only illustrates the most suitable for the surroundings of Coptidis Rhizoma, also helpful to further research and practice of cultivation regionalization, wild resource monitoring and large-scale cultivation of traditional Chinese medicine plants.